Implemented kill switches: 1
Kill Switch 1
Class
# Format: # Write how you would classify them.
Toxins
| Name | Number | Comment |
|---|---|---|
| KillerRed | chromoprotein activated by luminous irradiation - production of reactive oxygen species | |
| Barnase | bacterial protein with ribonuclease activity from Bacillus amyloliquefaciens- shows intense lethality |
Antagonists
| Name | Number | Comment |
|---|---|---|
| Zur | BBa_K1620004 | zinc uptake regulation protein - binds Zn2+ as a cofactor and negatively controls the promotor Zasp |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| Zasp | BBa_K1620001 | zinc activity sensing promoter element - active in the absense of zinc and Zur-dependent |
| RBS | BBa_B0030 | |
| KillerRed | chromoprotein activated by luminous irradiation - production of reactive oxygen species | |
| Barnase | bacterial protein with ribonuclease activity from Bacillus amyloliquefaciens- shows intense lethality | |
| Ter | BBa_B1010 | artificial terminator |
| pCons | BBa_J23101 | constitive promotor |
| Zur | BBa_K1620004 | zinc uptake regulation protein - binds Zn2+ as a cofactor and negatively controls the promotor Zasp |
| pUspA | BBa_K1620000 | stress promotor induced under several stress conditions |
| SmtA | BBa_K1620007 | Synechococcus metallothionenin A - able to bind heavy metal ions |